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rna sequencing transcriptome library  (Illumina Inc)


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    Structured Review

    Illumina Inc rna sequencing transcriptome library
    a Schematic illustration of the <t>RNA-seq</t> and metabolomics analyses. b Gene cluster trend in different groups ( n = 3 mice). c GO enrichment analysis of genes in cluster 1. d GO enrichment analysis of genes in cluster 3. e GO enrichment analysis of genes in cluster 4. GO enrichment analysis was performed using a one-sided hypergeo metric test. Terms with a p < 0.05 were considered significantly enriched. f Heatmap o f the relative abundance of proinflammatory cytokines and purine metabolic genes in the three groups ( n = 3 mice). g PCA score plot of different groups subjected to metabolic analysis. h Pathway enrichment analysis of the DEMs enriched in ALI mice treated with ND@Monos (vs. the ALI group). Significance of enrichment was determined by a hypergeometric test with an FDR-corrected p -value ( q -value) threshold of 0.05. i , j Western blot analysis and quantification of IL-1β, P-creb and P-P65 levels ( n = 5 mice). Data are presented as mean ± SD. NC group indicates normal mice, and PBS indicates mice treated with vehicle. Statistical analysis was performed by one-way ANOVA with Tukey’s multiple comparison test. Source data are provided as a Source Data file.
    Rna Sequencing Transcriptome Library, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 12799 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transcriptomic+libraries/TruSeq+RNA+Library+Prep/pmc12946169-402-1-16
    Average 99 stars, based on 12799 article reviews
    rna sequencing transcriptome library - by Bioz Stars, 2026-10
    99/100 stars

    Images

    1) Product Images from "A DNA-based nanodevice senses purinergic signaling and drives an immune switch for resolving inflammation"

    Article Title: A DNA-based nanodevice senses purinergic signaling and drives an immune switch for resolving inflammation

    Journal: Nature Communications

    doi: 10.1038/s41467-026-68879-2

    a Schematic illustration of the RNA-seq and metabolomics analyses. b Gene cluster trend in different groups ( n = 3 mice). c GO enrichment analysis of genes in cluster 1. d GO enrichment analysis of genes in cluster 3. e GO enrichment analysis of genes in cluster 4. GO enrichment analysis was performed using a one-sided hypergeo metric test. Terms with a p < 0.05 were considered significantly enriched. f Heatmap o f the relative abundance of proinflammatory cytokines and purine metabolic genes in the three groups ( n = 3 mice). g PCA score plot of different groups subjected to metabolic analysis. h Pathway enrichment analysis of the DEMs enriched in ALI mice treated with ND@Monos (vs. the ALI group). Significance of enrichment was determined by a hypergeometric test with an FDR-corrected p -value ( q -value) threshold of 0.05. i , j Western blot analysis and quantification of IL-1β, P-creb and P-P65 levels ( n = 5 mice). Data are presented as mean ± SD. NC group indicates normal mice, and PBS indicates mice treated with vehicle. Statistical analysis was performed by one-way ANOVA with Tukey’s multiple comparison test. Source data are provided as a Source Data file.
    Figure Legend Snippet: a Schematic illustration of the RNA-seq and metabolomics analyses. b Gene cluster trend in different groups ( n = 3 mice). c GO enrichment analysis of genes in cluster 1. d GO enrichment analysis of genes in cluster 3. e GO enrichment analysis of genes in cluster 4. GO enrichment analysis was performed using a one-sided hypergeo metric test. Terms with a p < 0.05 were considered significantly enriched. f Heatmap o f the relative abundance of proinflammatory cytokines and purine metabolic genes in the three groups ( n = 3 mice). g PCA score plot of different groups subjected to metabolic analysis. h Pathway enrichment analysis of the DEMs enriched in ALI mice treated with ND@Monos (vs. the ALI group). Significance of enrichment was determined by a hypergeometric test with an FDR-corrected p -value ( q -value) threshold of 0.05. i , j Western blot analysis and quantification of IL-1β, P-creb and P-P65 levels ( n = 5 mice). Data are presented as mean ± SD. NC group indicates normal mice, and PBS indicates mice treated with vehicle. Statistical analysis was performed by one-way ANOVA with Tukey’s multiple comparison test. Source data are provided as a Source Data file.

    Techniques Used: RNA Sequencing, Western Blot, Comparison

    Related Articles

    Construct:

    Article Title: Genome-scale revealing the central metabolic network of the fast growing methanotroph Methylomonas sp. ZR1.
    Article Snippet: Methylomonas sp. ZR1 was an isolated new methanotrophs that could utilize methane and methanol growing fast and synthesizing value added compounds such as lycopene.. In this study, the genomic study integrated with the comparative transcriptome analysis were taken to understanding the metabolic characteristic of ZR1 grown on methane and methanol at normal and high temperature regime.. Complete Embden-Meyerhof-Parnas pathway (EMP), Entner–Doudoroff pathway (ED), Pentose Phosphate Pathway (PP) and Tricarboxy Acid Cycle (TCA) were found to be operated in ZR1.

    Article Title: Integrated metabolome and transcriptome revealed the flavonoid biosynthetic pathway in developing Vernonia amygdalina leaves
    Article Snippet: .. In an effort to overcome this limitation, we constructed transcriptomic libraries of V. amygdalina leaves at 10, 20, and 30 DAG, and sequenced by Illumina HiSeqTM platform. ..

    Article Title: Effects of Methanol on Carotenoids as Well as Biomass and Fatty Acid Biosynthesis in Schizochytrium limacinum B4D1
    Article Snippet: Total RNA was extracted with TRIzol (Invitrogen). .. Transcriptomic libraries were constructed according to the manufacturer’s instructions (Illumina, San Diego, CA), and 2 × 150 bp paired-end sequencing was performed on the Illumina MiSeq platform. (ii) Transcriptomic analysis. .. The reference genome was Aurantiochytrium limacinum ATCC MYA-1381 ( https://genome.jgi.doe.gov/portal/AurlimATCMYA1381_FD/AurlimATCMYA1381_FD.info.html ).

    Sequencing:

    Article Title: Genome-scale revealing the central metabolic network of the fast growing methanotroph Methylomonas sp. ZR1.
    Article Snippet: Methylomonas sp. ZR1 was an isolated new methanotrophs that could utilize methane and methanol growing fast and synthesizing value added compounds such as lycopene.. In this study, the genomic study integrated with the comparative transcriptome analysis were taken to understanding the metabolic characteristic of ZR1 grown on methane and methanol at normal and high temperature regime.. Complete Embden-Meyerhof-Parnas pathway (EMP), Entner–Doudoroff pathway (ED), Pentose Phosphate Pathway (PP) and Tricarboxy Acid Cycle (TCA) were found to be operated in ZR1.

    Article Title: Transcriptome analysis provides new insights into leaf shape variation in birch
    Article Snippet: Key message The present study revealed many functional molecules for leaf splitting during the apical bud development and the switch development from the apical buds to leaf marginal tissues.. Abstract The lobe, an important character of leaf architecture, is present in Betula pendula ‘Dalecarlica’.. The presence of split lobes is of great significance in plant growth and development.

    Article Title: Effects of Methanol on Carotenoids as Well as Biomass and Fatty Acid Biosynthesis in Schizochytrium limacinum B4D1
    Article Snippet: Total RNA was extracted with TRIzol (Invitrogen). .. Transcriptomic libraries were constructed according to the manufacturer’s instructions (Illumina, San Diego, CA), and 2 × 150 bp paired-end sequencing was performed on the Illumina MiSeq platform. (ii) Transcriptomic analysis. .. The reference genome was Aurantiochytrium limacinum ATCC MYA-1381 ( https://genome.jgi.doe.gov/portal/AurlimATCMYA1381_FD/AurlimATCMYA1381_FD.info.html ).

    RNA Extraction:

    Article Title: SARS-CoV-2 variant spike and accessory gene mutations alter pathogenesis.
    Article Snippet: .. After RNA extraction as described above, transcriptomic libraries were generated and sequenced on an Illumina NovaSEq. .. 6000 (S4 flow cell, 100-bp paired-end; Illumina).

    Generated:

    Article Title: SARS-CoV-2 variant spike and accessory gene mutations alter pathogenesis.
    Article Snippet: .. After RNA extraction as described above, transcriptomic libraries were generated and sequenced on an Illumina NovaSEq. .. 6000 (S4 flow cell, 100-bp paired-end; Illumina).

    Single Cell:

    Article Title: Myeloid lineage C3 induces reactive gliosis and neuronal stress during CNS inflammation.
    Article Snippet: .. All transcriptomic libraries were sequenced on an Illumina NovaSeq 6000 at the Johns Hopkins Single Cell and Transcriptomics Core according to Illumina and 10X Genomics recommendations. ..

    Transcriptomics:

    Article Title: Myeloid lineage C3 induces reactive gliosis and neuronal stress during CNS inflammation.
    Article Snippet: .. All transcriptomic libraries were sequenced on an Illumina NovaSeq 6000 at the Johns Hopkins Single Cell and Transcriptomics Core according to Illumina and 10X Genomics recommendations. ..

    other:

    Article Title: Effects of Methanol on Carotenoids as Well as Biomass and Fatty Acid Biosynthesis in Schizochytrium limacinum B4D1
    Article Snippet: Transcriptomic 506 libraries were constructed according to the manufacturer’s instructions (Illumina, San Diego, CA), 507 and 2×150 bp paired-end sequencing was performed on the Illumina Miseq platform.



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    Image Search Results


    Changes in intestinal short chain fatty acids and spleen transcriptome in mice treated with SYD. (A) The boxplot of acetic acid abundance among three groups; (B) The boxplot of isobutyric acid abundance among three groups; (C) Differences in PISA scores among the three groups; (D) Differences in body weight among three groups of mice; (E) and (F) The difference in thickness between the left and right ears of mice among the three groups; (G) The correlation between short chain fatty acids and phenotype indicators in mice, yellow represents positive correlation, blue represents negative correlation, and darker colors indicate higher correlation coefficients; (H) The volcano plot of transcriptome between Psoriasis and SYD Treatment groups; (I) The heatmap of inflammation related genes among significant differentially expressed genes; (J) The dot plot of GO enrichment analysis of upregulated genes; (K) The GSEA analysis of the G protein-coupled receptor signaling pathway; (L) The analysis of CIBERSORT, which displayed the relative abundance of different cell subpopulations in three groups; (M) The box plot shows significant differences in the abundance of plasma, Treg, and Tfh cell subpopulations among the three groups.

    Journal: Frontiers in Microbiology

    Article Title: Sanyin decoction alleviates psoriasis by reshaping gut microbiota and modulating the gut–spleen–skin axis

    doi: 10.3389/fmicb.2026.1799928

    Figure Lengend Snippet: Changes in intestinal short chain fatty acids and spleen transcriptome in mice treated with SYD. (A) The boxplot of acetic acid abundance among three groups; (B) The boxplot of isobutyric acid abundance among three groups; (C) Differences in PISA scores among the three groups; (D) Differences in body weight among three groups of mice; (E) and (F) The difference in thickness between the left and right ears of mice among the three groups; (G) The correlation between short chain fatty acids and phenotype indicators in mice, yellow represents positive correlation, blue represents negative correlation, and darker colors indicate higher correlation coefficients; (H) The volcano plot of transcriptome between Psoriasis and SYD Treatment groups; (I) The heatmap of inflammation related genes among significant differentially expressed genes; (J) The dot plot of GO enrichment analysis of upregulated genes; (K) The GSEA analysis of the G protein-coupled receptor signaling pathway; (L) The analysis of CIBERSORT, which displayed the relative abundance of different cell subpopulations in three groups; (M) The box plot shows significant differences in the abundance of plasma, Treg, and Tfh cell subpopulations among the three groups.

    Article Snippet: High-quality RNA was subsequently used for transcriptome library construction using the MGIEasy Fast RNA Reagent (MGI, 940-002921-00), mRNA was enriched using oligo (dT) magnetic beads, followed by fragmentation with fragmentation buffer at a controlled temperature.

    Techniques: Clinical Proteomics

    Changes in transcriptome of mouse skin after SYD treatment. (A) The volcano of differentially expressed genes; (B) The GO bioprocess (BP) analysis of significant upregulated genes; (C) The KEGG analysis of significant upregulated genes; (D) The GO molecular function (MF) analysis of significant upregulated genes; (E)-(H) The GSEA enrichment of significant regulated genes, NES < 0, represents downregulated signaling pathway, adjusted p value < 0.05, represents statistically significant; (I) The GSEA enrichment of upregulated signaling pathway; (J) The result of skin immune infiltration between Psoriasis group and Treatment group using CIBERSORT; (K) Boxplot of significant differences in immune cell subpopulations (Macrophage_M2, Neutrophils, Dendritic resting_cells).

    Journal: Frontiers in Microbiology

    Article Title: Sanyin decoction alleviates psoriasis by reshaping gut microbiota and modulating the gut–spleen–skin axis

    doi: 10.3389/fmicb.2026.1799928

    Figure Lengend Snippet: Changes in transcriptome of mouse skin after SYD treatment. (A) The volcano of differentially expressed genes; (B) The GO bioprocess (BP) analysis of significant upregulated genes; (C) The KEGG analysis of significant upregulated genes; (D) The GO molecular function (MF) analysis of significant upregulated genes; (E)-(H) The GSEA enrichment of significant regulated genes, NES < 0, represents downregulated signaling pathway, adjusted p value < 0.05, represents statistically significant; (I) The GSEA enrichment of upregulated signaling pathway; (J) The result of skin immune infiltration between Psoriasis group and Treatment group using CIBERSORT; (K) Boxplot of significant differences in immune cell subpopulations (Macrophage_M2, Neutrophils, Dendritic resting_cells).

    Article Snippet: High-quality RNA was subsequently used for transcriptome library construction using the MGIEasy Fast RNA Reagent (MGI, 940-002921-00), mRNA was enriched using oligo (dT) magnetic beads, followed by fragmentation with fragmentation buffer at a controlled temperature.

    Techniques:

    a Schematic illustration of the RNA-seq and metabolomics analyses. b Gene cluster trend in different groups ( n = 3 mice). c GO enrichment analysis of genes in cluster 1. d GO enrichment analysis of genes in cluster 3. e GO enrichment analysis of genes in cluster 4. GO enrichment analysis was performed using a one-sided hypergeo metric test. Terms with a p < 0.05 were considered significantly enriched. f Heatmap o f the relative abundance of proinflammatory cytokines and purine metabolic genes in the three groups ( n = 3 mice). g PCA score plot of different groups subjected to metabolic analysis. h Pathway enrichment analysis of the DEMs enriched in ALI mice treated with ND@Monos (vs. the ALI group). Significance of enrichment was determined by a hypergeometric test with an FDR-corrected p -value ( q -value) threshold of 0.05. i , j Western blot analysis and quantification of IL-1β, P-creb and P-P65 levels ( n = 5 mice). Data are presented as mean ± SD. NC group indicates normal mice, and PBS indicates mice treated with vehicle. Statistical analysis was performed by one-way ANOVA with Tukey’s multiple comparison test. Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: A DNA-based nanodevice senses purinergic signaling and drives an immune switch for resolving inflammation

    doi: 10.1038/s41467-026-68879-2

    Figure Lengend Snippet: a Schematic illustration of the RNA-seq and metabolomics analyses. b Gene cluster trend in different groups ( n = 3 mice). c GO enrichment analysis of genes in cluster 1. d GO enrichment analysis of genes in cluster 3. e GO enrichment analysis of genes in cluster 4. GO enrichment analysis was performed using a one-sided hypergeo metric test. Terms with a p < 0.05 were considered significantly enriched. f Heatmap o f the relative abundance of proinflammatory cytokines and purine metabolic genes in the three groups ( n = 3 mice). g PCA score plot of different groups subjected to metabolic analysis. h Pathway enrichment analysis of the DEMs enriched in ALI mice treated with ND@Monos (vs. the ALI group). Significance of enrichment was determined by a hypergeometric test with an FDR-corrected p -value ( q -value) threshold of 0.05. i , j Western blot analysis and quantification of IL-1β, P-creb and P-P65 levels ( n = 5 mice). Data are presented as mean ± SD. NC group indicates normal mice, and PBS indicates mice treated with vehicle. Statistical analysis was performed by one-way ANOVA with Tukey’s multiple comparison test. Source data are provided as a Source Data file.

    Article Snippet: The RNA sequencing transcriptome library was generated with a TruSeq TM RNA sample preparation kit from Illumina (San Diego, CA). cDNA was synthesized via a SuperScript double-stranded cDNA synthesis kit (Invitrogen) with random hexamer primers (Illumina).

    Techniques: RNA Sequencing, Western Blot, Comparison